miR-197 Participates in Lipopolysaccharide-Induced Cardiomyocyte Injury by Modulating SIRT1

Figure 1

Effects of miR-197 on the viability and apoptosis of LPS-induced H9c2 cells. (a) The effect of LPS on H9c2 cell viability was detected by the CCK-8 assay. (b) The expression level of miR-197 in LPS-induced H9c2 cells was examined by qRT-PCR. U6 was used as the internal control. (c) To affirm the effect of the miR-197 inhibitor, qRT-PCR was applied to detect miR-197 expression level. U6 was used as the internal control. (d) The CCK-8 assay was conducted to examine the viability of LPS-induced H9c2 cells transfected with or without the miR-197 inhibitor. (e, f) Flow cytometry was performed to demonstrate the apoptosis of cells after LPS treatment or transfection. The experiments were repeated in triplicate. vs. control; ; vs. LPS + IC. LPS, lipopolysaccharide; CCK-8, cell counting kit-8; qRT-PCR, quantitative real-time polymerase chain reaction; I, miR-197 inhibitor; IC, inhibitor control.

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