miR-197 Participates in Lipopolysaccharide-Induced Cardiomyocyte Injury by Modulating SIRT1

Figure 4

miR-197 participated in LPS-induced cardiomyocyte injury by modulating SIRT1. (a, b) To affirm the effect of transfection with siSIRT1, a western blot was conducted to measure the protein level of SIRT1. GAPDH was used as the loading control. (c) CCK-8 was used to examine cell viability. (d) After transfection with siSIRT1, the LDH assay kit was employed to measure levels of LDH. (e, f) ELISA was applied for evaluating IL-1β and IL-6 levels. (g, h) Western blot was utilized to examine the protein levels of apoptosis-related genes Bcl-2, Bax, and cleaved caspase-3. The abovementioned experiments were repeated at least three times. ; vs. LPS; ; ; vs. LPS + I + siNC. CCK-8, cell counting kit-8; qRT-PCR, quantitative real-time polymerase chain reaction; LDH, lactate dehydrogenase; ELISA, enzyme-linked immunosorbent assay; SIRT1, silent information regulator 1; LPS, lipopolysaccharide; IL-1β, interleukin-1β; IL-6, interleukin-6; Bcl-2, B-cell lymphoma-2; Bax, BCL2-associated X; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; siSIRT1, small interfering RNA against SIRT1; I, miR-197 inhibitor; IC, inhibitor control; NC, negative control.

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