Research Article
Exogenous Hydrogen Sulfide Activates PI3K/Akt/eNOS Pathway to Improve Replicative Senescence in Human Umbilical Vein Endothelial Cells
Figure 4
Establishment of replicative senescence model in HUVECs. SA-β-Gal staining (a) and the statistics (b) of HUVECs at different CPDLs (100×). Senescent cells were stained blue. The CPDL 8, 16 and 30 groups showed a significant increase SA-β-Gal level as compared with the CPDL 2 group. n = 3 per group. , by LSD-t test after ANOVA. The expression of PAI-1 of HUVECs at different CPDLs as detected by western blot (c) and its statistics (d) The CPDL8,16, and 30 groups showed a significant increase of PAI- 1 protein expression compared to the CPDL 2 group. n = 3/group. , , as detected by LSD-t test after ANOVA.
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