Research Article

Exogenous Hydrogen Sulfide Activates PI3K/Akt/eNOS Pathway to Improve Replicative Senescence in Human Umbilical Vein Endothelial Cells

Figure 6

Impact of NaHS on replicative senescence of HUVECs. SA-β-Gal staining (a) and the statistics (b) of HUVECs in each group (100×). Senescent cells were stained blue. n = 3 per group. , by LSD-t test after ANOVA. The expression of PAI-1 of HUVECs in different groups as detected by western blot (c) and its statistics (d) n = 3 per group. , as examined by LSD-t test after ANOVA. CON: control group, HUVECs at CPDL2; RS: replicative senescence group, HUVECs at CPDL16; RS + NaHS: HUVECs at CPDL16 and exposed to 200 μmol/L NaHS for 24 hours.
(a)
(b)
(c)
(d)