Research Article

Exogenous Hydrogen Sulfide Activates PI3K/Akt/eNOS Pathway to Improve Replicative Senescence in Human Umbilical Vein Endothelial Cells

Figure 8

Effect of NaHS pre-treatment on NO concentration in HUVECs. Intracellular NO production was assessed using the nitric oxide fluorometric assay kit and the effects of NaHS on NO concentration in the indicated groups were compared in each group. n = 3 per group. , by LSD-t test after ANOVA. CON: control group, HUVECs at CPDL2; RS: replicative senescence group, HUVECs at CPDL16; RS + NaHS: HUVECs at CPDL16 and exposed to 200 μmol/L NaHS for 24 hours. RS + NaHS + LY:HUVECs at CPDL16 and exposed to 10 μmol/L LY294002 prior to treatment of 200 μmol/L NaHS for 24 hours.