Development and Validation of an ELISA at Acidic pH for the Quantitative Determination of IL-13 in Human Plasma and Serum

Table 5

Sensitivity, working range, and intra- and interrun accuracy and precision of the IL-13 assay under acidic conditions. Inter- and intrarun accuracy and precision were determined based on five levels of QCs ranging from the LLOQ to the ULOQ, assessed over six runs and five preparations per run.

Parameters	Performance
Sensitivity	0.59 pg/mL
Working range	LLOQ: 0.59 pg/mL; ULOQ: 68.4 pg/mL
Intrarun accuracy and precision	Accuracy: 84.6% to 116.2%
	Precision: 1.1% to 11.2%
Interrun accuracy and precision	Accuracy: 98.2 to 105.5%
	Precision: 5.0 to 12.6%