Monitoring of Serial Presurgical and Postsurgical Changes in the Serum Proteome in a Series of Patients with Calcific Aortic Stenosis

Figure 3

Increased levels of antithrombin-III and zinc-α-2-glycoprotein in postsurgical sera compared with those in presurgical sera of 14 CAS patients. Western blot analyses with anti-antithrombin-III antibody (a), anti-zinc-α-2-glycoprotein antibody (b), and anti-α-1B-glycoprotein antibody (c) in presurgical (pre) and postsurgical (post) sera of 14 patients (P6~P19) with CAS were performed. Arrow indicates reacted corresponding protein. Representative photos of two patients concerning each protein [patient numbers P8 and P12 for antithrombin-III in (a), P8 and P11 for zinc-α-2-glycoprotein in (b), and P12 and P15 for α-1B-glycoprotein in (c)] as well as a mixture (N) of 10 normal sera from control N5 to N14 are shown. To confirm equal levels of proteins per lane, nonspecific proteins stained with Coomassie Brilliant Blue are shown (CBB). The intensity of each band in presurgical and postsurgical sera of 14 patients and 10 normal control sera that reacted with each antibody was measured. Ratios of levels of each protein in presurgical and postsurgical sera from 14 patients and normal control sera from 10 volunteers compared with that in the mixture of 10 normal control sera (1.0) were determined by band intensity. Means ± SE of triplicate experiments were calculated, and statistical analysis between presurgical and postsurgical data was done with the paired t-test, whereas that between presurgical or postsurgical and normal control data was performed with the unpaired t-test. . .

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