Monitoring of Serial Presurgical and Postsurgical Changes in the Serum Proteome in a Series of Patients with Calcific Aortic Stenosis

Table 3

Proteins with differential levels in postsurgical and presurgical sera of the CAS patients.

Unused; ProtScore^a	%Coverage^b	Peptides^c (95%)	UniProt number	Gene symbol	Protein name	iTRAQ ratio^d; Average ± SE	value^e	Molecular function
14.0	76.2	10	P02735	SAA1	Serum amyloid A protein	8.377 ± 1.656	—^f	Acute-phase reactant
18.0	40.4	9	P02750	LRG1	Leucine-rich -2-glycoprotein	2.139 ± 0.224	0.0105	Unknown
83.2	73.7	93	P01009	SERPINA1	-1-antitrypsin	1.883 ± 0.290	0.0289	Protease inhibitor
46.7	52.2	30	P00751	CFB	Complement factor B	1.879 ± 0.219	0.0136	Complement
12.2	17.2	7	P02748	C9	Complement component C9	1.844 ± 0.191	0.0176	Complement
13.4	35.4	10	P02743	APCS	Serum amyloid P component	1.724 ± 0.195	—^f	Acute-phase reactant
38.3	52.8	19	P01008	SERPINC1	Antithrombin-III	1.679 ± 0.146	0.0060	Blood coagulation
11.0	19.0	7	Q06033	ITIH3	Inter--trypsin inhibitor heavy chain H3	1.622 ± 0.189	—^f	Hyaluronan binding
19.7	14.1	9	P01031	C5	Complement C5	1.609 ± 0.120	0.0042	Complement
39.0	33.9	24	Q14624	ITIH4	Inter--trypsin inhibitor heavy chain H4	1.605 ± 0.132	0.0152	Acute-phase reactant
116.0	61.6	72	P00450	CP	Ceruloplasmin	1.513 ± 0.045	0.0212	Transporter
32.4	57.3	21	P25311	AZGP1	Zinc--2-glycoprotein	1.509 ± 0.087	0.0275	Lipid catabolism
51.0	69.8	25	P02774	GC	Vitamin D-binding protein	1.327 ± 0.075	0.0435	Sequestration of actin
27.6	40.4	13	P04217	A1BG	-1B-glycoprotein	1.297 ± 0.078	0.0236	Unknown
27.1	38.8	17	P10909	CLU	Clusterin	1.284 ± 0.100	0.0390	Extracellular chaperone
71.3	28.0	35	P02751	FN1	Fibronectin	0.914 ± 0.116	—^f	Extracellular matrix

A score of protein confidence (ProtScore) for a detected protein that is calculated from the peptide confidence from spectra that are not already “used” by higher scoring proteins in the experiments.
^bThe percentage of matching amino acids from identified peptides.
^cThe number of distinct peptides with at least 95% confidence in the experiments.
^diTRAQ ratio of postsurgical sera compared with those in presurgical sera (postsurgical versus presurgical).
^eStatistical analysis of iTRAQ ratio of postsurgery/presurgery of patients’ sera compared with that of of control volunteers’ sera (patient versus control, Student’s -test).
^fProtein that did not appear in more than two control sera.