Toll-Like Receptor 9 Inactivation Alleviated Atherosclerotic Progression and Inhibited Macrophage Polarized to M1 Phenotype in ApoE^−/− Mice

Figure 2

Inactivation of TLR9 alleviated atherosclerotic vulnerability. Oil red O staining of lipids (a), immunostaining for CD68 positive macrophages (b) and α-actin (c), and sirius red staining for collagen (d) in brachiocephalic artery plaques. Bars represent mean ± SEM from 7 mice. compared with ApoE^−/− saline mice. Original magnification ×200.

(a) Oil red O

(b) CD68

(c) α-actin

(d) Sirius red