Dynamics of SOX2 and CDX2 Expression in Barrett’s Mucosa

Table 1

Primary antibodies and immunohistochemistry conditions used in this study.

Antibody	Clone	Antigen retrieval buffer	Antigen retrieval conditions	Dilution	Incubation time (min)	Localization	Source
CDX2	CDX2-88	Citrate buffer 10 mM pH 6.0	40 minutes at 98°C	1 : 50	Overnight (4°C)	Nuclear	Biogenex, San Ramon, CA
MUC2	PMH1	0.1 U/mL neuraminidase	2 h at 37°C	Undiluted	Overnight (4°C)	Cytoplasmatic	Supernatant [28]
MUC5AC	CLH2	None	None	1 : 10	Overnight (4°C)	Cytoplasmatic	Supernatant [28]
MUC6	CLH5	None	None	1 : 10	Overnight (4°C)	Cytoplasmatic	Supernatant [28]
SOX2	SP-76	EDTA 10 mM pH 8.0	40 minutes at 98°C	1 : 50	1 h (room temperature)	Nuclear	Cell Marque, Rockling, CA

Neuraminidase from Clostridium perfringens type VI (Sigma) was diluted in sodium acetate buffer (pH 5.5).