ELL2 Is Downregulated and Associated with Galactose-Deficient IgA1 in IgA Nephropathy

Figure 5

Gd-IgA1 production after knockdown ELL2 in DAKIKI cells. Western blot of ELL2 confirmed robust knockdown. Transfection of DAKIKI cells with shELL2 resulted in reduction in ELL2 protein expression. A significant increase in Gd-IgA1 levels was shown in DAKIKI cells transfected with shELL2 treatment. “Mock” indicates mock-transfected cells going through the transfection processes without addition of shRNA.