Identification of Serum CMTM2 as a Potential Biomarker for HBV-Related Disorders

Figure 4

Decreased CMTM2 expression in HBV-infected hepatic cells. The protein levels and cultural supernatants levels of CMTM2 in HepG2 cells and HepG2.2.15 cells were determined by western blot and ELISA (a, b). β-Actin was used as an internal control in Western blot. After transfected with HBV expressing plasmid pcDNA3.1-HBV (1.1 and 1.3) or empty plasmid pcDNA3.1 into HepG2 cell, both western blot and ELISA assay were used to measure protein levels and cultural supernatants levels (c, d). p <0.05; NS: no statistical significance.

(a)

(b)

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(d)