Identification of Serum CMTM2 as a Potential Biomarker for HBV-Related Disorders

Figure 5

HBx inhibited CMTM2 expression. After transfected with four HBV viral protein plasmids (HBp, HBc, HBs and HBx) or empty plasmid pcDNA3.1 into HepG2 cell, respectively, the protein levels of CMTM2 were detected using Western blot (a, b). After transfected with HBx plasmid or empty plasmid pcDNA3.1 into HepG2 cell, the mRNA level was determined by real-time PCR (c). Relative luciferase activities were measured according to standard procedures after that the CMTM2 promoter luciferase reporter and HBx or pcDNA3.1 plasmids were co-transfected into HepG2 cells (d). p <0.05; NS: no statistical significance.

(a)

(b)

(c)

(d)