A Trypanosoma cruzi Genome Tandem Repetitive Satellite DNA Sequence as a Molecular Marker for a LAMP Assay for Diagnosing Chagas’ Disease

Figure 3

Tc-LAMP specificity: visual examination of LAMP products by adding SYBR Green I. Lanes Tc: T. cruzi CL Brener or Dm28 strain DNA (0.5 ng/μL); lanes N: negative controls (ultrapure water, no DNA). Trematode DNA in lanes Sm (Schistosoma mansoni), Sh (S. haematobium), Fh (Fasciola hepatica), Am (Amphimerus sp.), Dd (Dicrocoelium dendriticum), and Ec (Echinostoma caproni). Nematode DNA in lanes Ax (Anisakis simplex), Bp (Brugia pahangi), Lo (Loa loa), Mp (Mansonella perstans), Sv (Strongyloides venezuelensis), and As (Ascaris suum). Cestode DNA in lanes Hd (Hymenolepis diminuta), Eg (Echinococcus granulosus), Tg (Taenia saginata), and Ts (T. solium). Protozoan DNA in lanes Gi (Giardia intestinalis), Cp (Cryptosporidium parvum), Eh (Entamoeba histolytica), Pm (Plasmodium malariae), and Pv (P. vivax). Leishmania donovani DNA in lane Ld (Leishmania donovani in triplicate). Leishmania infantum DNA in lane Li (Leishmania infantum in triplicate).