RETRACTED: Long Noncoding RNA AFAP1-AS1 Promotes Cell Proliferation and Metastasis via the miR-155-5p/FGF7 Axis and Predicts Poor Prognosis in Gastric Cancer

Figure 3

AFAP1-AS1 directly interacted with miR-155-5p and negatively regulated miR-155-5p. (a, b) The localization of AFAP1-AS1 was identified in BGC-823 and SGC-7901 cells with subcellular fractionation assay. (c) The putative interacting sequence between AFAP1-AS1 and miR-155-5p was obtained from bioinformatics analysis. (d, e) Dual-luciferase reporter assay further validated the combination between AFAP1-AS1 and miR-155-5p in BGC-823 and SGC-7901 cells. (f, g) AFAP1-AS1 and miR-155-5p were enriched in the Ago2-containing beads by RIP assay in BGC-823 and SGC-7901 cells. (h) miR-155-5p was overexpressed in GC tissues (, ). (i) The expression correlation between AFAP1-AS1 and miR-155-5p in GC tissues was analyzed. (j, k) The expression levels of AFAP1-AS1 in BGC-823 and SGC-7901 cells were detected after downregulation or upregulation of miR-155-5p using qRT-PCR. , , .

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