miR-106a-5p Functions as a Tumor Suppressor by Targeting VEGFA in Renal Cell Carcinoma

Figure 3

miR-106a-5p targets the 3-UTR of VEGFA. (a) Predicted binding positions among VEGFA and miR-106a-5p and mutant sites on VEGFA. (b) The dual-luciferase reporter assay helped identify reporter activity in HK2 and 786-O cells transfected with the indicated microRNA mimics and wild-type or mutant VEGFA 3-UTR. (c) Dual-luciferase reporter assay transfected with the indicated microRNA mimics. (d) Protein level from cell harvest from (c).

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