Research Article
CD82 Suppresses ADAM17-Dependent E-Cadherin Cleavage and Cell Migration in Prostate Cancer
Figure 5
CD82 interacted with ADAM17 and inhibited the metalloprotease activity of ADAM17. (a) C4-2 cells were transfected with siRNA of CD82, and the transcriptional expressions of ADAM17 were measured by real-time PCR. (b) 293T cell transfected with pcDNA-2xHA-CD82 and pcDNA-2xFlag-ADAM17 after 72 hours was harvested for immunoprecipitation to identify the interaction of CD82 and ADAM17. (c) CWR22Rv1 cells reexpressing CD82 were applied to immunoprecipitation. (d) The coexpression of CD82 and ADAM17 was analyzed by immunofluorescence staining assay in CWR22Rv1 cells reexpressing CD82. (e) ADAM17 metalloprotease activities were monitored by fluorogenic substrate assay in PCa cells with diverse CD82 expressions. (f) Schematic depicting the effect of CD82 in E-cadherin shedding in this study. . Error bars indicate s.d. from at least two technical replicates.
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