The Role of Fecal Fusobacterium nucleatum and pks⁺ Escherichia coli as Early Diagnostic Markers of Colorectal Cancer

Table 3

Clinical model and biomarker outcome prediction of CRC, CAP, and controls.

Test result variable(s)	AUC (95% CI)	value	Youden	Cut-off point	SEN (%)	SPE (%)	PPV	NPV	LR+	LR-
CRC vs. control
CEA	0.826 (0.73, 0.91)	<0.01	0.506	2.25	71.4	79.2	0.70	0.80	3.4	0.3
CA199	0.627 (0.51, 0.75)	0.051	0.194	5.52	57.1	62.3	0.56	0.64	1.5	0.6
F. nucleatum	0.735 (0.59, 0.87)	<0.01	0.431	1.13	69.2	73.9	0.67	0.75	2.6	0.4
pks	0.810 (0.67, 0.96)	<0.001	0.666	2.25	93.3	73.3	0.90	0.80	3.5	0.1
Panel 1	0.855 (0.72, 0.98)	<0.001	0.645	-	75.0	89.5	0.75	0.89	7.1	0.2
Panel 2	0.859 (0.63, 1.0)	<0.001	0.629	-	80.0	82.9	0.78	0.84	4.6	0.2
Panel 3	0.844 (0.71, 0.97)	<0.001	0.670	-	84.6	82.4	0.82	0.85	4.8	0.1
Panel 4	0.871 (0.66, 1.0)	<0.001	0.718	-	75.0	96.8	0.77	0.96	23.4	0.2
Panel 5	0.887 (0.68, 1.0)	<0.001	0.713	-	75.0	98.1	0.77	0.98	39.4	0.2
CAP vs. control
CEA	0.710 (0.57, 0.84)	0.006	0.391	1.90	71.4	67.7	0.67	0.72	2.2	0.4
CA199	0.764 (0.64, 0.88)	<0.001	0.421	3.23	67.9	74.2	0.67	0.75	2.6	0.4
F. nucleatum	0.741 (0.56, 0.91)	0.025	0.361	1.04	70.9	65.2	0.66	0.70	2.0	0.4
pks	0.818 (0.64, 0.98)	0.003	0.659	1.97	90.9	75.0	0.88	0.81	3.6	0.1
Panel 1	0.837 (0.70, 1.0)	0.032	0.575	-	85.1	72.4	0.81	0.78	3.0	0.2
Panel 2	0.720 (0.49, 0.94)	0.002	0.364	-	50.0	86.4	0.60	0.81	3.6	0.5
Panel 3	0.827 (0.62, 0.97)	<0.001	0.607	-	85.7	75.0	0.82	0.80	3.4	0.1
Panel 4	0.841 (0.51, 1.0)	<0.001	0.590	-	66.7	92.3	0.70	0.91	8.6	0.3
Panel 5	0.846 (0.57, 1.0)	<0.001	0.570	-	66.7	90.3	0.70	0.89	6.8	0.3
CRC vs. CAP
CEA	0.684 (0.51, 0.85)	0.05	0.329	2.05	90.3	42.9	0.69	0.76	1.5	0.2
CA199	0.440 (0.26, 0.61)	0.52	0.166	28.03	45.2	71.4	0.69	0.48	1.6	0.7
F. nucleatum	0.514 (0.33, 0.69)	0.88	0.071	3.59	64.5	42.9	0.61	0.46	1.1	0.1
pks	0.389 (0.21, 0.56)	0.23	-	2.67	48.4	42.9	0.54	0.37	0.8	1.2
Panel 1	0.486 (0.31, 0.66)	0.88	0.166	-	45.2	71.4	0.69	0.48	1.6	0.7
Panel 2	0.479 (0.30, 0.65)	0.82	0.166	-	45.2	71.4	0.69	0.48	1.6	0.7
Panel 3	0.560 (0.38, 0.73)	0.52	0.163	-	80.6	35.7	0.64	0.57	1.2	0.5
Panel 4	0.472 (0.31, 0.66)	0.92	0.205	-	41.9	78.6	0.33	0.73	1.9	0.7
Panel 5	0.488 (0.29, 0.64)	0.76	0.205	-	41.9	78.6	0.33	0.73	1.9	0.7

SPE: specificity; SEN: sensitivity; Youden: Youden index; LR: likelihood ratio OB: occult blood; . Clinical model panel 1: F. nucleatum+CEA+CA199; panel 2: pks⁺ E. coli+CEA+CA199; panel 3: F. nucleatum+pks⁺ E. coli+FOBT; panel 4: F. nucleatum+pks⁺ E. coli+CEA+CA199; panel 5: F. nucleatum+pks⁺ E. coli+CEA+CA199+FOBT.