Research Article
Derivation and Comprehensive Analysis of Aging Patterns in Patients with Bladder Cancer
Figure 9
Vitro validation. (a) Relative expression of CLIC3 mRNA in normal and tumor cell lines. (b) Relative expression of CLIC3 mRNA in UM-UC-3 cell lines transfected with si-CLIC3. (c) Relative expression of CLIC3 mRNA in 5637 cell lines transfected with si-CLIC3. (d) CCK8 assays in UM-UC-3 cell lines transfected with si-CLIC3. (e) CCK8 assays in 5637 cell lines transfected with si-CLIC3. (f) Western blot analysis of CLIC3, p-β-catenin, total-β-catenin, p-GSK3β, and total-GSK3β expression in UM-UC-3 cell line after transfection. (g) Western blot analysis of CLIC3, p-β-catenin, total-β-catenin, p-GSK3β, and total-GSK3β expression in 5637 cell line after transfection. , , .
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