Research Article
N-Acetylcysteine Slows Down Cardiac Pathological Remodeling by Inhibiting Cardiac Fibroblast Proliferation and Collagen Synthesis
Figure 3
NAC improves oxidative stress response after aortic constriction. (a) WB detected the expression of GPX1 and GPX3 and gray value analysis. GAPDH was used as an internal control. (b) qRT-PCR detected the expression of SOD1. (c) qRT-PCR detected the expression of SOD2. (d) qRT-PCR detected the expression of GPX1. (e) qRT-PCR detected the expression of GPX3. (f) The kit detected the serum SOD activity. (g) The kit detected the serum GPX activity. (h) The kit detected the serum GSH activity. (i) Flow cytometry detected the intracellular ROS levels. “” indicates the statistical difference from the control group, ; “#” indicates the statistical difference from the aortic constriction group, . SOD: superoxide dismutase; GPX: glutathione peroxidase; GSH: glutathione; ROS: reactive oxygen species.
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