N-Acetylcysteine Slows Down Cardiac Pathological Remodeling by Inhibiting Cardiac Fibroblast Proliferation and Collagen Synthesis

Figure 4

NAC inhibits the NF-κB signaling pathway. (a) MTT detected the CF activity. (b) The ³H proline incorporation method detected the cell total collagen synthesis function. (c) EdU staining detected the cell proliferation and percentage analysis of positive cells. (d) WB detected the expression of NF-κB, IκK-α, IκB-α, and gray value analysis. GAPDH is used as an internal control. (e) qRT-PCR detected the expression of NF-κB. (f) qRT-PCR detected the expression of IκK-α. (g) qRT-PCR detected the expression of IκB-α. “” indicates the statistical difference from the control group, ; “#” indicates the statistical difference from the aortic constriction group, .

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