USP1 Promotes GC Metastasis via Stabilizing ID2

Figure 1

Stable knockdown of USP1 inhibits GC metastasis both in vitro and in vivo. (a, b) mRNA and protein expression levels of USP1 in normal gastric mucosa cells and two human GC cells (GES-1, BGC-823, and MGC-803) were detected by RT-qPCR and Western blot; (c) the efficacy of shRNA1 and shRNA2 mediated knockdown of USP1 was detected by Western blot; (d, e) in vitro experiments, MGC-803 and BGC-823 cells were transfected with shUSP1#1 or shUSP1#2; the real-time cellular analysis (RTCA) were used to evaluate cell migration; (f) in vivo experiments, MGC-803 cells transfected with shUSP1 or shNC were injected into the tail veins of nude mice; the representative images of HE staining of liver tissues with metastatic cells and the number of mice with liver metastasis were shown (; ).

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