USP1 Promotes GC Metastasis via Stabilizing ID2

Figure 3

USP1 promotes GC metastasis by upregulating ID2 expression. (a, b) The mRNA and protein levels of ID2 in normal gastric mucosa cells and two human GC cells were detected by RT-qPCR and Western blot; (c) MGC-803 and BGC-823 cells were transfected with flag-vector or USP1-flag; the protein expression levels of USP1 and ID2 were detected by Western blot; (d) MGC-803 and BGC-823 cells were transfected with shNC or shUSP1; the protein expression levels of USP1 and ID2 were detected by Western blot; (e) BGC-823 cells were transfected with shUSP11 and/or his- ID2, the protein expression levels of USP1 and ID2 were detected by Western blot, and the effects of cell migration were detected by RTCA. (f) BGC-823 cells were transfected with flag-USP1 and/or shID2, the protein expression levels of USP1 and ID2 were detected by Western blot, and the effects of cell migration were detected by RTCA (; ).

(a)

(b)

(c)

(d)

(e)

(f)