Effects of Long Noncoding RNA HOTAIR Targeting miR-138 on Inflammatory Response and Oxidative Stress in Rat Cardiomyocytes Induced by Hypoxia and Reoxygenation

Figure 1

Effect of H/R on H9C2 cells. (a) qRT-PCR was used to detect the expression of IL-1β. (b) qRT-PCR was used to detect the expression of IL-6. (c) qRT-PCR was used to detect the expression of TNF-α. (d) WB was used to detect the expression of IL-1β, IL-6, and TNF-α. (e) ELISA was used to detect the content of IL-1β. (f) ELISA was used to detect the content of IL-6. (g) ELISA was used to detect the content of TNF-α. (h) ELISA was used to detect the content of MDA. (i) ELISA was used to detect the activity of SOD. (j) qRT-PCR was used to detect the expression of HOTAIR. (k) qRT-PCR was used to detect the expression of miR-138. (l) Immunofluorescence staining was used to detect the expression of SOD1 (magnification: 400x). (“” indicates statistical difference from the control group, ).

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