Research Article
MicroRNA-181b Serves as a Circulating Biomarker and Regulates Inflammation in Heart Failure
Table 2
All primers used in this study.
| | IL-1β | | F: 5-TGACCCATGTGAGCTGAAAG-3 | | R: 5-AGGGATTTTGTCGTTGCTTG-3 | | IL-6 | | F: 5-CAGGAACGAAAGTCAACTCCA-3 | | R: 5-ATCAGTCCCAAGAAGGCAACT-3 | | TNF-α | | F: 5-GCCCACGTCGTAGCAAAC-3’ | | R: 5-GCAGCCTTGTCCCTTGAA-3 | | miRNA-21 | | RT primer: GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACTCAACAT | | F: 5-GGGAGCTTATCAGACTGATG-3 | | R: 5-TGCGTGTCGTGGAGTC-3 | | miR-155 | | RT primer: GTCGTATCCAGTGCGTGTCGTGGAGTCGGCAATTGCACTGGATACGACACCCCTA | | F: 5-GGTGCTAATCGTGATAGGG-3 | | R: 5-AGTGCGTGTCGTGGAGTCG-3 | | miR-181b | | RT primer: GTCGTATCCAGTGCAGGGTCCGAGGTGCACTGGATACGACACCCACC | | F: 5-TGCGGAACATTCATTGCTGTC-3 | | R: 5-CCAGTGCAGGGTCCGAGGT-3 | | U6 | | RT primer: CGCTTCACGAATTTGCGTGTCAT | | F: 5-GCTTCGGCAGCACATATACTAAAAT-3 | | R:5-CGCTTCACGAATTTGCGTGTCAT-3 | | GAPDH | | F: 5-GCTTCGGCAGCACATATACTAAAAT -3 | | R: 5-CGCTTCACGAATTTGCGTGTCAT-3 |
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