miRNA-576 Alleviates the Malignant Progression of Atherosclerosis through Downregulating KLF5

Figure 3

KLF5 was the target gene binding miRNA-576. (a) Binding sequences in 3UTR of KLF5 and miRNA-576. (b) Luciferase activity in 293T cells cotransfected with KLF5 WT/KLF5 MUT and miRNA-576 mimics/negative control, respectively. RAW264.7 cells were pretreated with 200 mg/L ox-LDL for 24 h. The (c) mRNA and (d) protein levels of KLF5 in RAW264.7 cells transfected with miRNA-576 mimic or inhibitor. (e) Relative levels of IL-1, IL-6, and TNF-α in RAW264.7 cells transfected with miRNA-576 mimic or inhibitor.

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