E2F1-Induced lncRNA BAIAP2-AS1 Overexpression Contributes to the Malignant Progression of Hepatocellular Carcinoma via miR-361-3p/SOX4 Axis

Figure 5

Regulation relationship between BAIAP2-AS1 and miR-361-3p. (a) Relative BAIAP2-AS1 expressions in cytosolic and nuclear fractions of HepG2 and PLC5 cells. (b) Diagram of the BAIAP2-AS1 putative binding sites and corresponding mutant sites in BAIAP2-AS1 mRNA sequences. (c) Luciferase activity in HepG2 and PLC5 cells cotransfected with miR-361-3p mimics or miR-361-3p inhibitors and luciferase reporters containing BAIAP2-AS1-WT or BAIAP2-AS1-MT. (d) Biotin-coupled sense or antisense DNA probes targeting BAIAP2-AS1 were incubated with HepG2 and PLC5 cells lysate to pull down RNAs which were further analyzed using RT-qPCR for the amounts of BAIAP2-AS1 and miR-361-3p. (e) Biotin-labeled BAIAP2-AS1 RNA and antisense RNA were incubated with HepG2 and PLC5 cell lysate to pull down RNAs. (f) Dysregulation of miR-361-3p expression in BAIAP2-AS1 knockdown HepG2 and PLC5 cells. (g) RT-qPCR analysis of miR-361-3p expression in four HCC cells and LO2 cells. (h) RT-qPCR assays of the levels of BAIAP2-AS1 in HepG2 and PLC5 cells after overexpression or knockdown of miR-361-3p. and .

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