IL-38 and IL-36 Target Autophagy for Regulating Synoviocyte Proliferation, Migration, and Invasion in Rheumatoid Arthritis

Figure 2

IL-38 and IL-36 regulate synoviocyte proliferation, migration, and invasion. (a) SW982 cells transfected with indicated plasmids for 24 hours were incubated with EdU followed by fixation and permeabilization and then subjected to EdU staining with Apollo567 and nuclear staining with Hoechst 33258. Representative micrographs (200x) from three independent experiments are shown. (b) SW982 cells were transfected with the indicated constructs for 24 hours followed by scratching to form a linear wound with pipette tip. Cells were then cultured for indicated hours, and images of the wound were captured using a phase contrast inverted microscope with 100x magnification. (c) SW982 cells with IL-36 or IL-38 or empty vector transfection for 24 hours were digested by trypsin and resuspended and placed into upside of the transwell. Cells were cultured for 48 hours and were determined by crystal violet staining and microscopy (100x). Representative images are presented from at least three biological replicates.

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