IL-38 and IL-36 Target Autophagy for Regulating Synoviocyte Proliferation, Migration, and Invasion in Rheumatoid Arthritis

Figure 3

IL-38 and IL-36 modulate autophagy. (a) SW982 cells were transfected with IL-36 or IL-38 overexpression construct or empty vector for 24 hours. Cells were then subjected to immunofluorescent staining using LC3 antibody, and nucleus were labeled with DAPI. Representative micrographs (100x) from three dependent experiments were shown. (b) SW982 cells with the indicated plasmids transfection for 24 hours were harvested and lysed in RIPA buffer and subjected to SDS-PAGE and Western blot analysis. The endogenous LC3I/II turnover was assessed using LC3 specific antibody. GAPDH was detected as control. The data are representative of three independent experiments. , compared to the vector group.

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