circRNA hsa_circ_0005909 Predicts Poor Prognosis and Promotes the Growth, Metastasis, and Drug Resistance of Non-Small-Cell Lung Cancer via the miRNA-338-3p/SOX4 Pathway

Figure 3

circ_0005909 regulates miRNA-338-3p in NSCLC cells. (a) Schematic graph of the putative binding sites of miRNA-338-3p in the circ_0005909. (b) The expressions of miRNA-338-3p in tumor samples by RT-PCR. (c) Increased miRNA-338-3p expression is shown in four NSCLC cells, determined by RT-PCR. (d) miRNA-338-3p expressions are shown using TCGA datasets. (e) Luciferase reporter assay shows that the luciferase activity of either circ_0005909-WT was inhibited by miRNA-338-3p mimics. (f) Enrichment of circ_0006282 using the RNA pull-down experiment. (g) RT-PCR determined the levels of miRNA-338-3p in A549 and H460 cells after the transfection ().

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