circRNA hsa_circ_0005909 Predicts Poor Prognosis and Promotes the Growth, Metastasis, and Drug Resistance of Non-Small-Cell Lung Cancer via the miRNA-338-3p/SOX4 Pathway

Figure 5

SOX4 was a target of miRNA-338-3p. (a) The binding site of SOX4 3-UTR for miRNA-338-3p. (b) By searching TCGA datasets, the dysregulation of SOX4 was determined. (c) The levels of SOX4 in our cohort. (d) RT-PCR assays for the levels of SOX4 in four NSCLC cell lines and NHBE cells. (e) Luciferase reporter assay was used to measure the targeting relationship between miRNA-338-3p and SOX4. (f) RT-PCR assays and western blot assays for the levels of SOX4 in A549 and H460 cells transfected with miR-NC, miRNA-338-3p inhibitors, or miRNA-338-3p mimics. (g) SOX4 expression was determined in H460 and A549 cells transfected with vector, circ_0005909, or circ_0005909+miRNA-338-3p mimics ().

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