PFKFB4 Overexpression Facilitates Proliferation by Promoting the G1/S Transition and Is Associated with a Poor Prognosis in Triple-Negative Breast Cancer

Figure 2

PFKFB4 overexpression facilitates the proliferation of breast cancer cells by promoting the G1/S phase transition. (a) A WB assay revealed that 231 cells had elevated levels of HIF-1α and PFKFB4 following hypoxia treatment. Stable PFKFB4 overexpression (b) promoted the growth of TNBC cancer cells (c) and these cells exhibited more resistance to cisplatin (d) than negative control (231 NC) and empty vector (Vector) cells. (e, f, ) Overexpression of PFKFB4 remarkably promoted the formation of cell clones compared with control expression. (g, h) Flow cytometric analysis found that PFKFB4 overexpression increased the percentage of S-phase cells. Each bar represents the mean of three independent experiments (). (i) A WB assay showed increases in CDK6 and pRb Ser795 expression, which hinted that PFKFB4 overexpression might promote TNBC cell proliferation by facilitating the G1/S phase transition.

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