Validation of a One-Step Reverse Transcription-Droplet Digital PCR (RT-ddPCR) Approach to Detect and Quantify SARS-CoV-2 RNA in Nasopharyngeal Swabs

Table 2

Estimated Limit of Blank (LoB), Limit of Detection (LoD), and Limit of Quantification (LoQ) according to the guidelines of the International Conference on Harmonisation (ICH) [27].

Target			LOB	LOD	LOQ
E gene	0.54	0.98	2.1	7	10.3
N gene	0.75	2.02	4.1	13	21
RdRP gene	2.16	2.12	5.7	19.1	23.4
N2 gene	1.09	1.34	3.3	11	14.5
N3 gene	2.90	2.58	7.1	24.3	28.7