OATP1B1 Plays an Important Role in the Transport and Treatment Efficacy of Sorafenib in Hepatocellular Carcinoma

Figure 1

OATP1B1 expression in control and transgenic HepG2 and LO2 cells. The fluorescent photos show HepG2 and LO2 control cells and the cells after being transfected with lentivirus containing pGC-FU-OATP1B11a, pGC-FU-OATP1B11b, and pGC-FU-OATP1B115 (a, c). OATP1B1 expression is detected by Western Blotting and RT-qPCR. Compared to control GFP-HepG2 cells, OATP1B1 expression increases to approximately 110.41%, 147.92%, and 193.75% in OATP1B11a-GFP-HepG2, OATP1B11b-GFP-HepG2, and OATP1B115-GFP-HepG2 cells, respectively (b). Compared to control GFP-LO2 cells, OATP1B1 expression increases to approximately 32.93%, 45.12%, and 35.37% in OATP1B11a-GFP-LO2, OATP1B11b-GFP-LO2, and OATP1B115-GFP-LO2 cells, respectively (d). Protein level of OATP1B1 in HepG2 than LO2 cells was not obvious difference. NC: control.

(a) HepG2 cells and HepG2-OATP1B1 cells

(b) OATP1B1 expression in HepG2 cells and HepG2-OATP1B1 cells

(c) LO2 cells and LO2-OATP1B1 cells

(d) OATP1B1 expression in LO2 cells and LO2-OATP1B1 cells