OATP1B1 Plays an Important Role in the Transport and Treatment Efficacy of Sorafenib in Hepatocellular Carcinoma

Figure 7

(a) Compared with hematoxylin and eosin staining in normal liver tissue, hematoxylin and eosin staining showing severe congestion in hepatocellular carcinoma tissue, scattered inflammatory cells, nuclear pyknosis in most liver nuclei, and obvious mitosis. The black arrow indicates the mitotic image. (b) OATP2 expression in liver tissue of rats with or without treatment with PCN. Liver tissues were collected and washed with ice-cold PBS 3 times and lysed with lysis buffer. Lysis buffer was collected and centrifuged at  rpm for 20 min, and supernatants were used to analyze OATP2 levels by Western Blotting. Compared to the control rats, OATP2 expression is significantly increased in rats treated with PCN. (c) Western Blot bands show that OATP2 expression increases to approximately 227.7% of that in control rats. (d) mRNA expression of OATP2 and PXR in the experimental group is also significantly higher than in the control group. Symbols indicate rat models of hepatocellular carcinoma untreated (-) or treated (+) with PCN. ; .

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