FLT3 Amplification as Double Minute Chromosomes in a Patient with Chronic Myelomonocytic Leukemia

Figure 1

Array CGH, conventional cytogenetic, and FISH analyses with a FLT3/CON13 probe. Karyotype (a) and metaphase spread (b) showing 46,XY,i(17)(q10),del(20)(q11.2q13.3) 1~20dmin. (c) Array comparative genomic hybridization (CGH) was performed following the standard protocol provided by Agilent Technologies (Agilent Technologies, Santa Clara, CA, United States) showing the region of amplification. Equal quantities of labeled DNA products were mixed together and loaded onto Agilent’3 2400 k CGH chip, which is built based on GRCh37/hg19 with 1 kb median probe spacing. (d) FLT3/CON13 FISH probe. Interphase FISH (e) and metaphase FISH (f) showing numerous copies of FLT3.

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