ANTXR1 Regulates Erythroid Cell Proliferation and Differentiation through wnt/β-Catenin Signaling Pathway In Vitro and in Hematopoietic Stem Cell

Figure 2

ANTXR1 promotes erythroid cell proliferation and inhibits erythroid cell differentiation and apoptosis in cord blood CD34⁺ cells. (a) Western blot analysis was used to detect ANTXR1 levels after transfection of cord blood CD34⁺ cells with ANTXR1 overexpression vectors. (b) Quantification of western blots. (c) CCK8 assay of cord blood CD34⁺ cells proliferation at D6, D7, D8, and D9. (d) Flow cytometry detection of CD71 and CD235a expression in cord blood CD34⁺ cells during erythroid differentiation. (e) Wright-Giemsa staining results of cord blood CD34⁺ cells. (f) Flow cytometry to detect the expression of apoptotic cells in the differentiation of cord blood CD34⁺ cells. Error bars represent standard deviation of three independent experiments. , .

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