ANTXR1 Regulates Erythroid Cell Proliferation and Differentiation through wnt/β-Catenin Signaling Pathway In Vitro and in Hematopoietic Stem Cell

Figure 3

Interfering with ANTXR1 inhibits erythroid cell proliferation and promotes erythroid cell differentiation and apoptosis in K562 cells. (a) Western blot analysis was used to detect ANTXR1 levels after transfection of K562 cells with ANTXR1 interfering vectors. (b) Quantification of western blots. (c) CCK8 assay of K562 cells proliferation at 12 h, 24 h, 48 h, and 72 h. (d) Flow cytometry detection of CD71 and CD235a expression in K562 cells erythroid differentiation. (e) Benzidine staining-positive cells. (f) Changes in the proportion of benzidine-positive cells. (g, h) Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to determine the mRNA expression levels of GATA1 and ALAS2 in K562 erythrocytes induced by interfering with ANTXR1. (i) GATA1 and ALAS2 protein expression in K562 erythrocytes induced by interfering with ANTXR1. (j, k) Quantification of western blots. Error bars represent standard deviation of three independent experiments. , .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)