Disease Markers

Research Article

Overexpressed Thrombospondin 2 Induced Osteogenic Differentiation of Valve Interstitial Cells via Inhibition of Akt/NF-κB Signaling Pathway to Promote Calcific Aortic Valve Disease Development

Table 1

Primer sequences used for quantitative reverse transcription-polymerase chain reaction (qRT-PCR).
Target genePrimers, 5’-3’
THBS2Forward: ATAGACAGCTTCGCTCTGGAC
Reverse: CAAACCCCTGAAGTGACTCTC
OsteocalcinForward: GGCGCTACCTGTATCAATGG
Reverse: GTGGTCAGCCAACTCGTCA
Runx2Forward: TCAACGATCTGAGATTTGTGGG
Reverse: GGGGAGGATTTGTGAAGACGG
SPARCForward: CCCATTGGCGAGTTTGAGAAG
Reverse: CAAGGCCCGATGTAGTCCA
COL1A2Forward: GAGCGGTAACAAGGGTGAGC
Reverse: CTTCCCCATTAGGGCCTCTC
COL1A1Forward: GAGGGCCAAGACGAAGACATC
Reverse: CAGATCACGTCATCGCACAAC
SPP1Forward: CTCCATTGACTCGAACGACTC
Reverse: CAGGTCTGCGAAACTTCTTAGAT
CTGFForward: AAAAGTGCATCCGTACTCCCA
Reverse: CCGTCGGTACATACTCCACAG
MMP-2Forward: GATACCCCTTTGACGGTAAGGA
Reverse: CCTTCTCCCAAGGTCCATAGC
MMP-13Forward: TCCTGATGTGGGTGAATACAATG
Reverse: GCCATCGTGAAGTCTGGTAAAAT
AktForward: TCCTCCTCAAGAATGATGGCA
Reverse: GTGCGTTCGATGACAGTGGT
p65Forward: ATGTGGAGATCATTGAGCAGC
Reverse: CCTGGTCCTGTGTAGCCATT
GAPDHForward: CTGGGCTACACTGAGCACC
Reverse: AAGTGGTCGTTGAGGGCAATG