Upregulation of miR-664a-3p Ameliorates Calcific Aortic Valve Disease by Inhibiting the BMP2 Signaling Pathway

Figure 3

BMP2 is a target gene of miR-664a-3p. (a) Schematic representation of the predicted binding of miR-664a-3p to BMP2. Schematic diagram of the structure of the luciferase reporter system. (b) The relative luciferase activity of VICs coinfected with constructed luciferase reporters (BMP2-WT or BMP2-MUT) and miR-664a-3p mimic or inhibitor or negative control. (c) Western blotting analysis of BMP2 expression in VICs after alterations in the level of miR-664a-3p. Quantitative analysis of the protein bands are shown on the right. (d) Pearson’s correlation analysis between BMP2 and miR-664a-3p in human CAVs (five dots in the red box) and human non-CAVs (five dots in the blue box). The horizontal and vertical coordinates indicate the mean Ct values of miR-664a-3p and BMP2 in RT-qPCR, respectively. , , and .

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