Upregulation of miR-664a-3p Ameliorates Calcific Aortic Valve Disease by Inhibiting the BMP2 Signaling Pathway

Figure 5

miR-664a-3p regulates aortic valve calcification via Bmp2 in calcific aortic valve disease mice. (a, b) RT-qPCR was performed to measure the miR-664a-3p and Bmp2 expression after upregulation of miR-664a-3p and Bmp2 separately or simultaneously in the aortic valves of mice. (c) Bmp2, Runx2, Mst2, and Osterix expressions were detected by western blotting after the upregulation of miR-664a-3p and Bmp2 levels separately or simultaneously in the aortic valves of mice. Quantitative analyses of the protein bands are shown on the right. (d) HE staining of the aortic valve of mice in different groups. Scale bar, 400 μm. , , and .

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