[Retracted] PSMB5 Alleviates Ulcerative Colitis by Inhibiting ROS-Dependent NLRP3 Inflammasome-Mediated Pyroptosis

Figure 5

LPS-medicated decreased PSMB5 was related to the activation of NLRP3 inflammasome and ROS. PSMB5 expression at protein level (a) and mRNA level (b) was detected by Western blot and RT-PCR assay in siRNA targeting PSMB5 transfected HIEC-6 cells. (c) Western blotting for PSMB5 expression under LPS and Z-VAD-FMK conditions. (d) Western blot analysis of PSMB5, cleaved caspase-1 p20, pro-IL-1β, IL-1β, pro-IL-18, IL-18, NLRP3, and ASC expressions under LPS and Z-VAD-FMK conditions or LPS and MCC950 conditions. All data are expressed as mean ± SD ( =5). and ^#.

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