LINC01140 Targeting miR-452-5p/RGS2 Pathway to Attenuate Breast Cancer Tumorigenesis

Figure 1

LINC01140 overexpression suppressed BC cell proliferation and migration and facilitated apoptosis in vitro. (a) RT-qPCR was utilized for measuring LINC01140 expression in normal MCF-10A cells and BC cells (HCC1937, MCF-7, and MDA-MB-231). vs MCF-10A. (b) RT-qPCR was utilized for measuring LINC01140 level in BC tissues and adjacent tissues. . (c) Subcellular localization was utilized for measuring LINC01140 expression in cytoplasmic and nuclear of HCC1937 and MDA-MB-231 cells. (d) HCC1937 and MDA-MB-231 were transfected with LINC01140 overexpression vector (LINC01140-OE) and its corresponding negative control (empty vector). LINC01140 expression in these transfected cells was measured by RT-qPCR. vs empty vector. (e) Cell viability in HCC1937 and MDA-MB-231 cells delivered empty vector and LINC01140-OE, as uncovered utilizing CCK-8 assay. vs empty vector. (f) Migrated cells were counted utilizing transwell in HCC1937 and MDA-MB-231 cells delivered empty vector and LINC01140-OE. vs empty vector. (g) Bax and Bcl-2 protein levels was uncovered utilizing western blotting analysis in HCC1937 and MDA-MB-231 cells delivered empty vector and LINC01140-OE. vs empty vector.

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