LINC01140 Targeting miR-452-5p/RGS2 Pathway to Attenuate Breast Cancer Tumorigenesis

Figure 3

LINC01140 sponged miR-452-5p (a) StarBase was utilized for the prediction of miR-452-5p binding sites on LINC01140. (b) Luciferase reporter experiment was conducted to measure the luciferase activities among MDA-MB-2312 and HCC1937 cells that had a combined transfection of a miR-452-5p mimic or miR-NC plus either a LINC01140-WT or LINC01140-MUT reporter plasmid. vs mimic-NC. (c) MiR-452-5p and LINC01140 enrichment levels on anti-Ago2 bind magnetic beads, as determined via RIP assay. vs anti-IgG. (d) RT-qPCR was utilized for measuring miR-452-5p expression in BC as well as adjacent tissues. . (e) The association between the expressions of miR-452-5p and LINC01140 in BC tissues was analyzed by Pearson analysis. (f) RT-qPCR was utilized for the determination of miR-452-5p expression in normal MCF-10A cells and BC cells (HCC1937 and MDA-MB-231). vs MCF-10A. (g) RT-qPCR was utilized for measuring miR-452-5p expression in empty vector, mimic-NC, LINC01140-OE (OE), mimic or OE+mimic transfected HCC1937, and MDA-MB-231 cells. vs empty vector; ^^ vs mimic-NC; ## vs OE+mimic.

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