LINC00460 Stimulates the Proliferation of Vascular Endothelial Cells by Downregulating miRNA-24-3p

Figure 3

LINC00460 bound to miR-24-3p. (a) Relative levels of miRNA-24-3p, miRNA-485-5p, miRNA-149-5p, miRNA-662, and miRNA-671-5p in ECs transfected with pcDNA-NC or pcDNA-LINC00460. (b) MiR-24-3p level in ECs treated with 100 μg/mL ox-LDL for 0, 24, 48, and 72 h. (c) Binding sites in promoter regions of LINC00460 and miR-24-3p. (d) Luciferase activity in ECs after cotransfection with miR-24-3p mimic/negative control and LINC00460-WT/LINC00460-MT, respectively. (e) Transfection efficacies of miR-24-3p mimics and miR-24-3p inhibitor. (f) LINC00460 level in ECs transfected with NC, miR-24-3p mimics, or miR-24-3p inhibitor.

(a)

(b)

(c)

(d)

(e)

(f)