Research Article
HEIH Promotes Malignant Progression of Gastric Cancer by Regulating STAT3-Mediated Autophagy and Glycolysis
Figure 2
In vitro, HEIH promotes the proliferation, migration, and invasion of gastric cancer cells. (a) Transfection efficiency test results. (b) CCK8 method to detect the proliferation of MGC-803 and BGC-823 cell lines transfected with HEIH siRNA. (c) Assess cell proliferation through colony formation experiments. (d) Assess aggressiveness through Transwell analysis. (e) qRT-PCR detects the expression level of Twist1 in MGC-803 and BGC-823 cell lines after transfection. (f) qRT-PCR detects the expression level of Snail in MGC-803 and BGC-823 cell lines after transfection. (g) qRT-PCR detects the expression level of Slug in MGC-803 and BGC-823 cell lines after transfection. (h) qRT-PCR detects the expression level of N-cadherin in MGC-803 and BGC-823 cell lines after transfection. (i) qRT-PCR detects the expression level of E-cadherin in MGC-803 and BGC-823 cell lines after transfection. and .
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