Involvement of RUVBL1 in WNT/β-Catenin Signaling in Oral Squamous Cell Carcinoma

Figure 7

Knock-down of RUVBL1 by siRNA attenuated growth and metastasis of the OSCC cell line, H157, by inhibiting β-catenin signaling. (a) Knock-down of RUVBL1 by siRNA suppressed H157 cell proliferation as indicated using the colony formation assay. The box plot showed the number of cells. (b) Knock-down of RUVBL1 by siRNA inhibited migration of the H157 cells in the wound healing assay. Significant differences between groups were assessed using a Student’s test and shown by and ^#, . (c). After transfection of the H157 cells with RUVBL1 siRNA or scramble siRNA (siNC), cell invasive ability was determined by cell invasion assay. All experiments were performed in triplicate. Significant differences between groups were assessed using a Student’s test and shown by and ^#, . (d) Expression of β-catenin pathway proteins was detected by western blot analysis. Intensity of the gray band was calculated by ImageJ and shown in the figure.

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