Involvement of RUVBL1 in WNT/β-Catenin Signaling in Oral Squamous Cell Carcinoma

Figure 8

Knock-down of RUVBL1 by siRNA attenuated growth and metastasis of the OSCC cell line, Cal-27, by inhibiting β-catenin signaling. (a) Knock-down of RUVBL1 by siRNA inhibited migration of the Cal-27 cells in the wound healing assay. Significant differences between groups were assessed using a Student’s T test and shown by and #, p <0.05. (b). After transfection of the Cal-27 cells with RUVBL1 siRNA or scramble siRNA (siNC), cell-invasive ability was determined by cell invasion assay. All experiments were performed in triplicate. Significant differences between groups were assessed using a Student’s test and shown by and ^#, . (c) Expression of β-catenin pathway proteins was detected by western blot analysis. Intensity of the gray band was calculated by ImageJ and shown in the figure.

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