Research Article
Echium amoenum L. Ethanol Extract Protects Retinal Ganglion Cell after Glutamate and Optic Nerve Crush Injury
Figure 3
Neuroprotective effects of E. amoenum ethanol extract in the optic nerve crush (ONC) mouse model. (a) Retinas from different groups were harvested on day 7 after ONC, and flat-mounted retinas were subjected to immunostaining with RBPMS and Tuj1 antibodies. (b) Quantification of the number of RGCs in 1-mm2 retina sections. (c) Expression of the optic nerve protein NF-L determined by immunoblotting. (d) Quantification of the NF-L levels. Relative protein levels were calculated using ImageJ software. Results were obtained from three independent experiments. μm. Data are ; , , and . RGC: retinal ganglion cell; ECA: E. amoenum ethanol extract.
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