lncRNA LEF1-AS1 Acts as a Novel Biomarker and Promotes Hypopharyngeal Squamous Cell Carcinoma Progression and Metastasis by Targeting the miR-221-5p/GJA1 Axis

<div>Effect of LEF1-AS1 on cell proliferation and apoptosis. (a, b) Efficiency of LEF1-AS1 overexpression plasmid (a) or LEF1-AS1 siRNAs (b) in FaDu cells. (c, d) GSEA analysis of P53 (c) and apoptosis (d) gene sets based on LEF1-AS1 expression information in TCGA. (e, f) CCK8 analysis in LEF1-AS1 overexpressed (e) or silenced FaDu cells (f). (g) Colony formation assay. (h) Relative colony numbers in (g). (i) Apoptosis of LEF1-AS1 overexpressed or silenced FaDu cells were detected by flow cytometry. (j) Apoptotic cell percent in (i). (k) Protein levels of apoptosis markers including cleaved caspase 3, Bax, and Bcl-2 in LEF1-AS1 overexpressed or silenced FaDu cells. 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Disease Markers

fig2

Figure 2

Figure 2: lncRNA LEF1-AS1 Acts as a Novel Biomarker and Promotes Hypopharyngeal Squamous Cell Carcinoma Progression and Metastasis by Targeting the miR-221-5p/GJA1 Axis 

Figure 2 | lncRNA LEF1-AS1 Acts as a Novel Biomarker and Promotes Hypopharyngeal Squamous Cell Carcinoma Progression and Metastasis by Targeting the miR-221-5p/GJA1 Axis 